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Identification of unknown isolates using the 16S rRNA sequence

The sequence of the variable region of the 16S rRNA ribosomal gene from your submitted microbes is analyzed to identify their taxonomy, usually to the species level. Compared to phenotype-based approaches, the 16S-based identification approach is fast, accurate, convenient and reproducible, well suited for identifying and tracking unknown samples.

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The 16S-based identification process

• Single colony samples can be submitted in different formats from live cultures to just DNA.

• DNA is isolated from live samples if required.

• PCR amplification of the target region of the 16S rRNA gene.

• The PCR amplicon is sequenced using the 1492R primer. Request a Quote for sequencing from the forward primer or both forward and reverse primers.

• Sequence data is viewed and analyzed.

• Phylogenetic analysis identifies the closest neighbors in the Ribosomal Database Project (RDP) reference database.

• Taxonomic information is derived from proximity to the nearest reference.

Report and analysis results

Your analysis results are returned to as a package containing the following.

• Taxonomic Classifications

An Excel file listing the taxonomic classification of each of your samples based on the nearest neighbors in the RDB database.

• Phylogenetic Tree

A combined phylogenetic tree showing all of your strains in relation to each other and to the nearest known neighbors

• Sequence information

A file of all your 16S sequences in FASTA format